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. 2018 Feb 23;8:3521. doi: 10.1038/s41598-018-21942-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BI-2536 and BI-6727 reactivate HIV-1 provirus in the latently infected model cells. (a) ACH2 and U1 cells were cultured in the presence of 0.1% DMSO, BI-2536 or BI-6727 at the indicated concentrations for 16 hours. HIV-1 gag mRNA expression was measured by quantitative RT-PCR. Fold induction of gag mRNA normalized by gapdh mRNA expression was determined and compared with DMSO-treated cells. Each value is shown as a mean ± SD of triplicate determinations. (b) ACH2 and U1 cells were cultured in 0.1% DMSO only or in the presence of BI-2536 or BI-6727 at the indicated concentrations for 24 hours. Cell viability was measured by a WST-8 assay and shown as a percentage of that of DMSO control cells. Each value is shown as a mean ± standard deviation (SD) for triplicate samples. (c) ACH2 and U1 cells were incubated in the presence of 0.1% DMSO, 10 ng/mL PMA, 1 μM BI-2536, or 1 μM BI-6727 for 24 hours. Cell lysates were immunoblotted with anti-HIV-1 p24 and anti-α-tubulin antibodies. The arrows indicate bands of viral p24, p41, and p55. The cropped images of the blots are shown. The uncropped images are shown in Supplementary Fig. 2.