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. 2018 Feb 23;8:3549. doi: 10.1038/s41598-018-21552-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Allopurinol-treated mice are partially protected against hindlimb unloading-induced inflammation and oxidative stress. (A) Representative western blotting of soleus proteins (full-length blots are included in supplementary information. See Supplementary Figure 2). (B) Shows plasma MDA levels measured by HPLC. (C and D) show the densitometric analysis quantified by using relative expression in arbitrary units of MnSOD (C), and p-p38 (D). (E and F) Show NF-κB p65 binding activity (E), and plasma 6-keto prostaglandin F1α (F) Determined by ELISA. Actin was used as a loading control. Data are shown as mean ± SD. C (n = 4–7), U (n = 4–6), and UA (n = 4–6).