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. 2018 Jan 29;128(3):931–943. doi: 10.1172/JCI97229

Figure 5. Increased viral protein in neurons of microglia-depleted brains.

Figure 5

Mice were treated with PLX5622 for 7 days prior to intracranial infection with MHV. (A) Viral N protein and IBA1 immunostaining of the brainstem and (B) olfactory bulb in control and PLX5622-treated mice on day 5 p.i. (C) NeuroTrace and N protein staining of the brainstem and olfactory bulb on day 5 p.i. and quantification of N protein–positive neurons. (D) NeuroTrace and N protein staining of brainstem on day 5 p.i. Yellow arrow indicates an N protein–positive, NeuroTrace-negative cell; white arrows indicate double-positive cells. Original magnification, ×20 (A–C) and ×40 (D). Images are representative of 4 to 5 mice.