Figure 7. Macrophages are functionally altered by PLX5622 treatment.

Mice were treated with PLX5622 for 7 days prior to intracranial MHV infection. (A and B) Decreased MHC II (A) and increased Ly6C (B) levels on microglia and macrophages from PLX5622-treated mice on day 7 p.i. Data represent the mean ± SEM. (C) Macrophages were isolated from 3 mouse brains per group on day 4 p.i. and subjected to microarray analysis. A heatmap was generated from all genes with significant differences in expression, with 235 genes differentially expressed (log₂ expression values are shown from blue to red). (D) Fold change of selected differentially expressed genes in macrophages after PLX5622 treatment. Data in A and B are representative of 3 independent experiments, with 3 to 5 mice per group. *P < 0.05 and ****P < 0.0001, by Mann-Whitney U test.