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. 2018 Feb 12;128(3):1087–1105. doi: 10.1172/JCI97794

Figure 1. High bone mass and skeletal abnormalities in mice lacking Vhl in osteoblast lineage cells.

Figure 1

(A) Tibia length at 12 weeks (n = 4–7 per genotype). (B) Representative 3D micro-CT reconstructions of tibias from 12-week-old mice. (C) Bone volume relative to tissue volume (BV/TV, in %) determined by micro-CT at the indicated ages (n = 3–5/group). (D and E) Micro-CT analysis of the trabecular (D) and cortical (E) tibia regions at 12 weeks (n = 3–5), showing BV/TV; trabecular number (Tb.N), separation (Tb.Sp), and thickness (Tb.Th); and cortical thickness (Cort.Th) and porosity (Cort.Por). (F) Representative transverse micro-CT section of the tibia. (G) Vertebral BV/TV determined by micro-CT (n = 4). (H) Relative (Rel.) mRNA levels of Vegf and Epo in full bones of 12-week-old mice (n = 7). (I) Tibia histology showing H&E staining, PECAM-1 IHC for blood vessels (including magnifications), and reticulin-positive fibers (black stain and yellow arrow) indicative of BM fibrosis in Vhl cKO bones. Scale bars: 500 μm (H&E; PECAM-1 left), 50 μm (PECAM-1 right; reticulin). Graphs represent mean ± SEM, and *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test between genotypes.