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. 2018 Feb 12;128(3):1087–1105. doi: 10.1172/JCI97794

Figure 4. PN–Vhl cKO mice recapitulate the key skeletal and systemic features of the constitutive Vhl cKO model while showing normal BW and fat mass.

Figure 4

(A) Scheme of doxycyclin administration to silence Osx-Cre:GFP activity from conception until weaning. (B) BW of control and PN–Vhl cKO mice at the indicated ages (n = 6–9). (C) Abdominal fat weight as percentage of BW at 24 weeks (n = 6–7). (D) Serum adiponectin levels at 24 weeks (n = 7–8). (E) Relative BV/TV change over time analyzed by in vivo micro-CT. (F and G) Bone mineral density (BMD) (F) and BV/TV (%) (G) determined by ex vivo micro-CT at 24 weeks (n = 7–8). (H) Representative sirius red–stained tibia sections (scale bars: 500 μm) (n = 3). (I) Blood glucose levels at 3 weeks of age (overnight fast, n = 6), 12 weeks (overnight fast, n = 6–8), 15 weeks (3-hour fast, n = 6–8), and 24 weeks of age (overnight fast, n = 7–8). (J) GTT at 12 weeks (n = 3–7) and 24 weeks (n = 7–8), with corresponding AUC calculations. (K) Serum insulin levels in random-fed conditions at 24 weeks in females (left, n = 4–5) and males (right, n = 7). (L) ITT and corresponding AUC (n = 4–5). Graphs represent mean ± SEM, and *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test between genotypes, unless indicated otherwise. All data in B–J and L were obtained in male control and induced PN–Vhl cKO mice. Corresponding data from female groups are shown in Supplemental Figure 8.