Figure 6. Low-dose and high-dose atropine were equally effective at blocking HRV.

(A and B) Poincaré plots demonstrated greater short-term and long-term HRV (SD1 and SD2, respectively) in an Scn1a^R1407X/+ mouse compared with a WT mouse. (C) High-dose atropine (1 mg/kg) reduced HRV in an Scn1a^R1407X/+ mouse, consistent with blockade of parasympathetic output. (D) Low-dose atropine (0.03 mg/kg) was equally effective in reducing HRV in an Scn1a^R1407X/+ mouse. The plots in the 4 parts of this figure are from 4 individual animals. Summary data for replicate animals for these experiments are shown in Supplemental Figure 7. Number of replicates for each group was 7 (A), 10 (B), 6 (C), and 6 (D).