Figure 6. Reduced lipotoxic sphingolipids in HFD Lrp1-βKO islets.

Sphingolipids were assayed by mass spectrometry in pancreatic islets isolated from mice after doxycycline treatment and 8 months of HFD and normalized against the protein content of islet samples. n = 6 (control) and n = 4 (Lrp1-βKO) samples with 50 islets per sample. Sphingolipid species are categorized as (A) ceramides, (B) dihydro-ceramides, (C) lactosyl-ceramides, and (D) hexosyl-ceramides. The furthest left columns in every panel represent the sum of all species evaluated. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01 for Lrp1-βKO versus control by 2-tailed unpaired Student’s t test. ND, not detected.