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. 2018 Feb 19;128(3):1178–1189. doi: 10.1172/JCI97702

Figure 8. PPARγ2 overexpression impairs GSIS and insulin signaling in β cells after HFD.

Figure 8

(A) Doxycycline-inducible overexpression of FLAG-tagged PPARγ2 in mouse β cells. After 4 months of HFD, both Pparg2-βOE and control mice were switched to doxycycline HFD. Mice were subjected to oral glucose tolerance test (OGTT) (2 mg/g body weight) before and 2 weeks after doxycycline HFD. (B and C) Plasma glucose (B) and insulin (C) during oral glucose tolerance test. n = 4 (control) and n = 7 (Pparg2-βOE) mice. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01 for Pparg2-βOE versus control mice; #P < 0.05; ##P < 0.01 for before versus after doxycycline by 2-tailed unpaired Student’s t test. (D) Representative Western blots of insulin-signaling molecules with islets from mice after 3 weeks of doxycycline HFD.