Skip to main content
. 2018 Feb 12;128(3):1057–1073. doi: 10.1172/JCI96329

Figure 4. Blockade of IDO1/AhR pathway abrogates IFN-β–induced dormant TRCs.

Figure 4

(A and B) B16 or A375 TRCs were seeded in soft 3D fibrin gels. Two days later, IFN-β was added for a further 2 days or 4 days of culture in the presence or absence of DMF. The colony size (A) was presented relative to the PBS (day 2) group, which was set to 1, and colony number (B) was counted. Scale bars: 50 μm. (C) B16 or A375 TRCs were treated with IFN-β (5 ng/ml) with or without DMF for 48 hours. Cell apoptosis was determined by flow cytometry. (D and E) B16 or A375 TRCs were treated with PBS, IFN-β, 1-MT, or IFN-β + 1-MT for indicated times. The colony size was measured (D), and colony number was counted (E). Scale bar: 50 μm (D). (F and G) B16 or A375 TRCs were treated with IFN-β (5 ng/ml), 1-MT, IFN-β + 1-MT, or IFN-β + 1-MT + Kyn (200 μM) for 48 hours. Cell apoptosis was determined by flow cytometry. Data are from 3 independent experiments. Graphs represent mean ± SEM. **P < 0.01, 1-way ANOVA (A–G).