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. 2018 Feb 23;8:3542. doi: 10.1038/s41598-018-21505-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Barrier function is disrupted in MECs expressing the S²⁸⁸ variant of ZnT2. (a) Representative confocal images of E-cadherin (green) and ZnT2-HA (red) in MECs transfected to express wild-type ZnT2 (T²⁸⁸) or the ZnT2 variant (S²⁸⁸). Nuclei were counterstained with DAPI (blue; scale bar, 10 µm). (b) Representative immunoblot of zonula occludens-1 (ZO-1) in total cell lysates from MECs expressing T²⁸⁸ or S²⁸⁸. Ponceau staining served as a loading control. Cropped blots are displayed and full-length blots can be found in Supplementary Fig. S5a,b. (c) Quantification of ZO-1 expression. Data represent mean ZO-1 signal intensity ± SD from n = 3 samples/genotype; p < 0.05*. (d) Assessment of barrier function in cells expressing T²⁸⁸ and S²⁸⁸. Data represent mean FITC-dextran fluorescence (arbitrary units) ± SD, from n = 3 samples/genotype; the experiment was repeated two times. Mock represents mock-transfected MECs. Means with different letters are significantly different, p < 0.05.