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. 2018 Feb 23;8:3541. doi: 10.1038/s41598-018-21894-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impaired visual projection was induced by maternal ZIKV infection. (a) Schematic depiction of the anterograde tracing method used. (b) Brain atlases indicate the sites of brain slices in (c), (d), and (e), respectively. (c–e) Results of visual projection in the LGN (c), SC (d), and OX (e), respectively. (f) Mean gray value of visual projection fibers in the DLG, VLGM, SC, and OX. DLG and VLGM: n^Mock = 10, n^ZIKV = 14 samples; SC: n^Mock = 11, n^ZIKV = 10 samples; OX: n^Mock = 11, n^ZIKV = 9 samples from three independent experiments. Data are presented as the mean ± SEM. Student’s t-test, *p < 0.05, ***p < 0.001.