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. 2018 Feb 23;8:3565. doi: 10.1038/s41598-018-21895-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Purity and viability of IgM⁺ B cells sorted by magnetic activated cell sorting (MACS). (a) Upon sorting, the mean percentages of IgM⁺ cells from HK, PB and spleen (n = 3 for each tissue) were analysed by flow cytometry. The circle (○) represents total percentage of viable cells before gating for IgM⁺ events. Histogram represents one representative individual for each tissue, where IgM⁺ events are presented by the transparent peak and non-stained events by the black peak. (b) Viability of IgM⁺ cells kept in culture with or without CpG for 0, 12 and 24 hours. (c and d) The relative expression of MARCO and csf-1r in MACS and FACS sorted IgM⁺ cells, and in macrophage-like cells (MLC).