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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Vet Comp Oncol. 2017 Apr 11;16(1):55–68. doi: 10.1111/vco.12311

Figure 4.

Figure 4

Induction of apoptosis in C2 and NI-1 cells by JAK2- and STAT5-targeting drugs. C2 (A) and NI-1 cells (B) were incubated in control medium (Co) or medium containing various concentrations of JAK2- and STAT5 blockers at 37°C for 24 or 48 hours (h). Thereafter, cells were examined by flow cytometry using an antibody against Annexin V and propidium iodide (PI) staining. Results show the percent of all apoptotic cells (percentage of cells stained single-positive for Annexin V plus percentage of cells stained double-positive for Annexin V and PI) and represent the mean ± SD of 3 independent experiments. *P < .05. The effects of the dimethyl sulfoxide (DMSO) control did not differ from the medium control (not shown).