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. 2018 Feb 23;13(2):e0192693. doi: 10.1371/journal.pone.0192693

Fig 2. NAC and NF-κB inhibitor decreased CRIF1 deletion-induced expression of VCAM-1 in HUVECs.

Fig 2

Endothelial HUVECs were pretreated with NAC and NF-κB inhibitor (BAY11-7082) for 1 h and then transfected with CRIF1 siRNA (50, 100 pmol) for 48 h. (A) VCAM-1 protein expression was detected by western blotting and quantified by densitometric analysis. (B) The location and protein expression of VCAM-1 (red) and Hoechst (blue) in HUVECs were examined by immunofluorescence. Scale bars indicate 20 um. All western blots are representative of three independent experiments. The data are presented as means ± SEM of three independent experiments. *p < 0.05 vs. control cells. #p < 0.05 vs. CRIF1 siRNA cells.