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. 2018 Feb 23;13(2):e0192693. doi: 10.1371/journal.pone.0192693

Fig 4. SIRT1 mediated CRIF1 deficiency-induced NF-κB activation in HUVECs.

Fig 4

(A) HUVECs were transfected with CRIF1 siRNA (50 and 100 pmol) and SIRT1 siRNA (50 and 100 pmol) for 48 h, and the cytosolic and nuclear fractions were isolated. The protein expression levels of cytosolic p65 and IκBα and nuclear p65 were detected by western blotting. p65 and IκBα protein levels were quantified by densitometric analysis (right panel). (B) HUVECs were infected with β-Gal and SIRT1 adenovirus for 24 h, followed by transfection with CRIF1 siRNA (100 pmol) for 36 h. Cytosolic p65 and IκBα and nuclear p65 protein expression levels were quantified by densitometric analysis (right panel). All western blots are representative of three independent experiments. The data are presented as means ± SEM of three independent experiments. #p < 0.05 vs. CRIF1 control cells. *p < 0.05 vs. SIRT1 control cells.