A. Frequency of Foxp3+ (Treg) cells among CD4+ T cells of each genotype in the spleen and peripheral lymph nodes (PLN) of mixed bone marrow (BM) chimeric mice generated by transferring 1:1 ratio of bone marrow cells from 45.1+ (SJL) WT and 45.2+ DKO mice or control 45.1+ (SJL) WT and 45.2+ WT mice. B. Fold changes in the MFI of Treg-associated markers (GITR, ICOS, and CCR6) on 45.2+ compared to 45.1+ Treg cells in the PLNs of BM chimeric mice. Data are pooled from two independent experiments with at least three mice in each group. Each symbol represents an individual mouse (n=7-8). Error bars indicate the mean ± s.e.m., *P<0.05, **P<0.01, ***P<0.001 (unpaired, two-tailed Student’s t-test). ns, not significant.