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. 2017 Dec 12;10(2):290–303. doi: 10.1080/19420862.2017.1405203

Figure 2.

Figure 2.

Antibody a-fucosylation rate in host cell lines with GnT-III/Man-II double transfection. A: A-fucosylation level after transient transfection of glycoengineered host cell lines with mAb2 expression plasmid. Antibody was purified by protein A chromatography. The a-fucose ratio was determined by MALDI-TOF MS. The bars in grey represent the one-fold glycoengineered parental host cell lines. The corresponding HCLs that were glycoengineered a second time are depicted in black. CHO-K1 cells were used as control to determine the background of non-glycoengineered HCL (white bar). Clones selected for further evaluation are marked by asterisks. B: A-fucosylation level of mAb2 expressed in clones obtained by stable transfection of two-fold glycoengineered host cell lines. Clones derived from HCLs 17–483, 17–494 and 51–201 were cultivated in 6-well plates. Clones derived from HCLs 64–30, 64–6024, 64–6123, 260–501 and 260–1513 were cultivated in 24-well plates. Antibody was purified by protein A HPLC. The a-fucose ratio was quantified by MALDI-TOF MS.