Figure 3.

Overview of the development process and the lineage of the glycoengineered host cell lines. Glycoengineered HCLs were generated in a two-step strategy, starting with generating parental HCLs by stable transfection of wild-type CHO-K1 cells (highlighted by blue circle) with GnT-III and Man-II (highlighted by light orange circles). In a second step, parental HCLs were stably transfected a second time with GnT-III and Man-II (highlighted by orange circles). Generated glycoengineered CHO-K1 cell lines were subsequently used to establish stable cell lines (highlighted by green circle) for the expression of a-fucosylated mAbs. The numbers represent the clone naming as listed in Table 1.