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. 2017 Dec 12;10(2):290–303. doi: 10.1080/19420862.2017.1405203

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© 2018 The Author(s). Published by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — Characterization of transgene stability. A: GnT-III expression for each HCL was determined by flow cytometry after intracellular staining. Each time point was measured in triplicates. Relative GEO Mean: Geometric mean fluorescence intensity normalized to 100% at day zero. B: Man-II expression assessment by flow cytometry as described for GnT-III (A). C: Copy number determination at day zero and after 180 days (HCL 17–494) or 134 days (HCL 60–30) by qPCR. Error bars represent standard deviation.