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. 2017 Dec 12;10(2):290–303. doi: 10.1080/19420862.2017.1405203

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© 2018 The Author(s). Published by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Influence of medium trace elements on a-fucosylation degree of antibodies. Different concentrations for manganese, copper, and iron in a chemically-defined medium A (CDM-A) were used for cultivation experiments with preglycoengineerd CHO cells all expressing the same mAb3 (A). The relative biological activity (RBA, measured by FcγRIIIa chromatography) for mAb3 produced with clone 1 in a 14-day fed-batch cultivation with CDM-A variants A1 to A8 is shown in (B). Six mAb3 expressing clones, clone 1, clone 2, clone 3 - HCL 17–494 based, clone 4, clone 5, clone 6 - HCL 64–30 (as listed in Table 2) based were cultivated in a seven day batch process with low (A1) and high (A2) manganese levels. The RBA is shown in (C). The different glycan species of mAb3 produced with clone 1 in a 14-day fed-batch cultivation with CDM-A variants A1 to A8 are shown in (D).