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. 2017 Dec 14;10(2):304–314. doi: 10.1080/19420862.2017.1409320

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — FMAT binding of BBB targeting hIgG1TM to brain capillary endothelial cells. FMAT binding assay to confirm the binding of BBB targeting hIgGs identified from epitope competition bEND.3 phage selections. FMAT assay binding curves of BBB hIgG1, BBB (○) and eight BBB targeting hIgGs, BBBt0626 (•), BBBt0632 (▾), BBBt0726 (▴), BBBt0654 (♦), BBBt0717 (◊), BBBt0727 (x), BBBt0732 (+), BBBt0754 (□) and isotype control hIgG1 (▪). (A) BBB targeting hIgGs binding to mouse brain endothelial (bEND.3) cells (B) BBB targeting hIgGs binding to Rat Brain Capillary Endothelial Cells and (C) BBB targeting hIgGs binding to human D3 Brain Capillary Endothelial Cells.