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. 2017 Dec 14;10(2):304–314. doi: 10.1080/19420862.2017.1409320

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Indirect immunocytochemistry of lead BBB targeting hIgGs in bEND.3 cells. Images show fixed and permeabilized bEND.3 cells grown on collagen I-coated 96-well plates and stained with (A) BBB-IgG, (B) BBBt0626, (C) BBBt0632 and (D) Isotype control (NIP228). A secondary detection antibody alone control is shown in (E). Images are merged with Hoechst staining to show the position of the cell nuclei and are representative of 3 cultures, with four fields of view taken from each individual preparation of cells using the 20 × objective on an ImageXpress Micro XLS Wide Field High-Content Analysis System.