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. Author manuscript; available in PMC: 2019 Feb 23.
Published in final edited form as: Hepatology. 2018 Jan 2;67(2):750–761. doi: 10.1002/hep.29483

Figure 4. Role of Jagged 1 in bile duct development.

Figure 4

A) Jagged 1 expression in cells surrounding CK19-positive bile duct structures inside the liver organoids after 3 weeks differentiation (inset, a higher magnification of a ductal structure). B) Human FLPCs, seeded in liver ECM discs, were differentiated for 3 weeks in liver differentiation media (LDM) and LDM containing DAPT. The relative proportions of the different stages of bile duct formation were quantified and are presented as percent of total ductal structures. C) RT-PCR analysis of the expression of transcription factors regulating bile duct morphogenesis hepatic nuclear factor 6 (HNF6) and SOX9 and of mature markers of cholangiocytes AE2 and GGT1. D) NICD and CK19 expression in organoids treated with DAPT and control. * = p<0.05