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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Curr Opin Neurobiol. 2017 Aug 30;48:17–29. doi: 10.1016/j.conb.2017.08.003

Figure 2. Visualization of intact circuits using hydrogel-based clearing methods.

Figure 2

A. A mouse brain before and after clearing with PARS and long-term storage in RIMS (adapted from [71]). The sample demonstrates moderate tissue expansion due to acrylamide embedding. B. Confocal image of dopaminergic neurons in the SNc (red) and cholinergic afferents from the pedunculopontine tegmental nucleus (green) visualized in a 1mm-thick PACT-cleared section. C–D. Whole brain imaging of fluorescently-labelled cells in Thy1-eYFP mice using light sheet microscopy.