A) Linear Discriminant Analysis provided 90.91% separation accuracy between HAD-1 and LE rats, and 100% separation accuracy between HAD-1 and LAD-1 rats based on 50 – 55 kHz FM USV data. No clear separation was observed between LE and LAD-1 rats. B) Linear Discriminant Analysis based on 22 – 28 kHz USV data Showed 100% separation between HAD-1 and LE rats, 95.45% separation between LAD-1 and LE rats, and 100% separation accuracy between HAD-1 and LAD-1 rats. C) Linear Discriminant Analysis based on 50 – 55 kHz FM USV data did not show any clear separation between the P, LE and NP rat lines. D) Linear Discriminant Analysis based on 22 – 28 kHz USV data Showed 92.31% separation between P and LE rats, 95.00% separation between NP and LE rats, and 100% separation accuracy between P and NP rats.