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. Author manuscript; available in PMC: 2019 Feb 28.
Published in final edited form as: J Control Release. 2018 Jan 3;272:9–16. doi: 10.1016/j.jconrel.2017.12.032

Figure 4.

Figure 4

Representative images of a tumor section and image analysis strategy. (a) 35 nm AuNPs (black) were visualized by silver enhancement and brightfield microscopy. (b) Blood vessels were stained with anti-CD31 antibody and fluorescent secondary antibody (green) and imaged by fluorescence microscopy for the same field of view. Images are shown before (a, b) and after (c, d) thresholding. (e) Composite image showing relative locations of AuNPs (cyan) and blood vessels (green) in a tumor section, with red lines indicating the shortest distance from each AuNP to a blood vessel.