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. 2018 Jan 31;175(6):953–967. doi: 10.1111/bph.14137

Figure 3.

Figure 3

Effects of IW‐1973 on hepatic fibrosis. (A) Representative photomicrographs (200× magnification) of liver sections stained with Masson's trichrome, Sirius Red and a specific α‐SMA antibody used for the assessement of hepatic fibrosis in mice receiving chow diet (n = 10), CDAAH diet (n = 15), CDAAH plus sGC stimulator IW‐1973 at 1 mg·kg−1 (n = 10) or CDAAH plus IW‐1973 at 3 mg·kg−1 (n = 10). (B) Histomorphometric analysis of the area stained with Masson's trichrome, Sirius Red and α‐SMA. (C) Hepatic COL1A1, COL1A2, MMP2, TGF‐β1 and TIMP‐1 mRNA expression determined by real‐time PCR. Results are expressed as mean ± SEM. Scale bar = 50 μm.