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. 2018 Jan 1;145(1):dev159905. doi: 10.1242/dev.159905

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — Wnt5a gain of function enhances vascular specification of Nkx2.5-expressing CPCs. (A-C) Flk1-GFP;Nkx2.5^Cre;Rosa^tdTom mice were crossed with the Wnt5a^GOF strain. Whole-mount and coronal views of control and Wnt5a^GOF hearts at E12.5 (A), and the percentage of NkxECs (B) and frequency of phospho-histone H3-positive cells per field (C) were quantified in control and Wnt5a^GOF hearts. (D-F) Flk1-GFP;Nkx2.5^Cre;Rosa^tdTom mice were crossed with the Wnt5a^flox/flox strain. Whole-mount and coronal views of Wnt5a^WT/flox and Wnt5a^flox/flox hearts at E13.5 (D), and the percentage of NkxECs (E) and frequency of phospho-histone H3-positive cells per field (F) were quantified in Wnt5a^WT/flox and Wnt5a^flox/flox hearts. Error bars represent s.d. between five biological replicates; in C,F counting 1 out of every 12 sections (10 µm thickness). Colocalization threshold masks denoting NkxECs (red) and nECs (green) are shown (A,D), with merged fluorescence images shown in the inset. Regions within dashed boxes (A,D) are magnified to the right. Asterisks indicate septal defects in mutant mice. Scale bars: 100 µm.