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. 2018 Jan 1;145(1):dev154468. doi: 10.1242/dev.154468

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Accumulation of autophagy markers in Asna1^Panc−/− DPE. (A) Immunohistochemistry of dorsal pancreatic sections from E13.5 Asna1^Panc:Ctrl and Asna1^Panc−/− embryos (n=5) using antibodies against p62 (green). Arrowheads indicate p62⁺ puncta. (B) Dorsal pancreatic sections from E13.5 Asna1^Panc:Ctrl and Asna1^Panc−/− embryos on a LC3-GFP transgenic background, showing LC3-GFP fluorescence in green (n=2). (C) Immunohistochemistry of dorsal pancreatic sections from E14.5 Atg5^Panc+/− and Atg5^Panc−/− embryos (n=3), using antibodies against p62 (green), E-cadherin (E-cad; green) and cleaved caspase3 (c.Casp.3; red). Insets show magnification of selected trunk (t) and acinar (a) epithelia areas (orange squares, letters and arrows). DAPI (blue) indicates nuclei in A-C. Dashed lines delineate pancreatic epithelium (e) and mesenchyme (m). Scale bars: 5 µm in A,B and insets in C; 50 µm in C. All image pairs were captured using the same settings.