Fig. 3.

Postnatal hydrocephalus in Ccdc39^prh/prh mice. (A-H) Nissl staining of P1 (A-F) and P5 (G,H) mutant and control brains. Development of the cerebral cortical layers was comparable between Ccdc39^wt/wt (B) and Ccdc39^prh/prh mutant (E) brain, whereas postnatal neural VZ/SVZ progenitors were significantly reduced in the Ccdc39^prh/prh mutant at P1 (C,F) and nearly absent at P5 (G,H). (I) Cerebral ventricular size in E18 and P1 brains. (J) P1 mutant brain had significantly smaller VZ/SVZ than control littermate. *P<0.05, **P<0.01 and ***P<0.001. (K) Western blotting of whole brain lysate from P10 brains. Reduced MBP and increased GFAP proteins indicated hypomyelination and enhanced gliosis in the mutant brains as compared with control littermates. ERK1/2, loading control. d-, dorsal; l-, lateral; dl-, dorsolateral.