Figure 2.

Effect of toll-like receptor 3 activation on IRFs. intestinal epithelial cells were transfected with or without Poly I:C at indicated concentrations for 3 or 6 h. (A,C) For mRNA quantification, total cellular RNA was collected at 3 h post-transfection and subjected to the qRT-PCR. (B,D) For protein quantification, cellular proteins were collected at 6 h post-transfection and subjected to immunoblot. β-actin serves as the loading control. P-IRF3:Phospho-IRF3; P-IRF7:Phospho-IRF7. Data shown were the mean ± SD of three independent experiments. Asterisks indicate that the differences are statistically significant (**P < 0.01).