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. 2018 Feb 19;9:247. doi: 10.3389/fimmu.2018.00247

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Copyright © 2018 Guo, Xu, Zhou, Zhou, Wang, Li, Liu, Liu, Zhang and Ho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of exosomes and delivery of intestinal epithelial cells (IECs) exosomes (Exo) to macrophages. (A) The expression of exosomal markers (Lamp2, Alix, CD63), and nonexosomal markers (EEA1, Cytochrome C, GAPDH) was determined by immunoblot. (B) The uptake of IECs exosomes labeled with PKH67 by macrophages. Macrophages were incubated with PHK67-labeled IECs exosomes (green) for 24 h and then stained with PKH26 for general cell membrane labeling (red) and Hoechst 33342 (blue) for nuclei. Data are representative of three independent experiments. Scale bar, 100 µm.