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. 2018 Feb 19;12:40. doi: 10.3389/fncel.2018.00040

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Copyright © 2018 Dubový, Klusáková, Hradilová-Svíženská, Joukal and Boadas-Vaello.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative medullary sections showing rostral ventromedial medulla (RVM) from naïve and sham-operated controls (A,B) and 3 weeks after sCCI or CSNT (C,D). The pictures illustrate increased intensity and expansion of GFAP immunostaining in RVM after sCCI and CSNT in comparison to RVM from naïve or sham-operated rat. Scale bars = 90 μm. (E) The drawing illustrates dissection of RVM samples for western blots including the nucleus raphe magnus and gigantocellularis pars alpha (triangle). (F) The top panel illustrates representative western blot of GFAP protein in RVM of naïve and sham-operated rats and 3 weeks after sCCI or CSNT. *Indicates a statistically significant difference (p < 0.05) compared to the value from sham-operated rats.