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. 2018 Feb 19;12:40. doi: 10.3389/fncel.2018.00040

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Copyright © 2018 Dubový, Klusáková, Hradilová-Svíženská, Joukal and Boadas-Vaello.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative sections illustrating OX42 immunofluorescence staining in dlPAG (A–D) and vlPAG (E–H) of naïve, sham-, sCCI- and CSNT-operated rats. Insets show the typical morphological types of OX42+ microglial cells from the respective animal groups. Scale bars = 90 μm. (I) Western blot analysis of OX42 (CD11b/c) protein. The top panel illustrates representative western blot in PAG of naïve and sham-operated rats and rats 3 weeks after sCCI or CSNT. (i) indicates ipsilateral and (c) contralateral segments of dorsolateral (dl) and ventrolateral (vl) PAG. Graph below the blot illustrates density data obtained from three blots after normalization to actin, expressed as fold-change relative to those of sham-operated rats (set as 1). *Indicates a statistically significant difference (p < 0.05) compared to the value from sham-operated rats.