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. 2018 Feb 19;9:269. doi: 10.3389/fimmu.2018.00269

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Copyright © 2018 Iula, Keitelman, Sabbione, Fuentes, Guzman, Galletti, Gerber, Ostrowski, Geffner, Jancic and Trevani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of autophagy in interleukin-1β (IL-1β) and pro-IL-1β secretion. Concentrations of IL-1β (A) and pro-IL-1β (B) in culture supernatants (secreted) and cell pellets (intracellular) of neutrophils stimulated or not with LPS + ATP for 5 h in the absence or presence of 3-methyladenine (3-MA) (5 mM) added 30 min before LPS stimulation. Concentrations of IL-1β (C) and pro-IL-1β (D) in culture supernatants (secreted) and cell pellets (intracellular) of mock-treated or starved neutrophils stimulated with LPS + ATP for 5 h. Data represent the mean ± SEM of four (A,B) or five (C,D) experiments performed in duplicate. Two-way ANOVA followed by Bonferroni’s multiple comparisons test.* p < 0.05 vs. basal or between LPS + ATP-treated or not with 3-MA.