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Recommended media and equipment needed to process clinical samples ahead of their implantation into mice. Requirements vary depending on tissue sample and chosen processing modality. Saline solution: 0.9% Sodium Chloride. Tissue Culture Media: DMEM or RPMI-1640, 10% fetal bovine serum (FBS) and antibiotics. Freezing media: 10% DMSO, at least 20% FBS in DMEM. Abbreviations: PBS, phosphate buffer saline; ACK lysis buffer, ammonium chloride potassium lysis buffer.
