Table 1.
Target region | Primer sequence (5́ → 3́) | Size of product | Annealing Temperature | References |
---|---|---|---|---|
intI-1F | TCATGGCTTGTTATGACTGT GTAGGGCTTATTATGCACGC |
600 bp* | 57̊C | 26 |
intI-1R | ||||
intI-2F | GATGCCATCGCAAGTACGAG CGGGATCCCGGACGGCATGCACGATTTGTA |
750 bp* | 57̊C | 27 |
intI-2R | ||||
intI-3F | GCCTCCGGCAGCGACTTTCAG ACGGATCTGCCAAACCTGACT |
650 bp* | 59̊C | 28 |
intI-3R |
bp = base pair, F = forward sequence, R = reverse sequence.