Table 1.
Nucleotide sequences used as primers and TaqMan probes for identification of Beijing and non-Beijing strains (18)
| Primers/probe names for target strains | Target | Sequence |
|---|---|---|
| nBjF | Non-Beijing strains | 5′-AAGCATTCCCTTGACAGTCGAA-3′ |
| nBjR | 5′-GGCGCATGACTCGAAAGAAG-3′ | |
| nBjTM | 5′-6FAM-TCCAAGGTCTTTG-MGB-NFQ-3′a | |
| BjF | Beijing strains | 5′-CTCGGCAGCTTCCTCGAT-3′ |
| BjR | 5′-CGAACTCGAGGCTGCCTACTAC-3′ | |
| BjTM | 5′-YAK-AACGCCAGAGACCAGCCGCCGGCT-DB-3′b |
a
MGB-NFQ, minor groove binding, nonfluorescent quencher.
b
YAK-DB, Yakima yellow-DABCYL (nonfluorescent dark quencher).