TABLE 1.
In vivo mcp promoter activities in WT and ΔvisN/R, Δrem, and ΔflbT mutant strains
| Plasmida (lacZ fusion) | β-Galactosidase activityb (Miller units) for strain: |
|||
|---|---|---|---|---|
| RU11/001c (WT) | RU11/814 (ΔvisNR) | RU11/555 (Δrem) | RU13/110 (ΔflbT) | |
| pRU2728 (mcpT) | 42 | 7 | 7 | 35 |
| pRU2283 (mcpU) | 235 | 0 | 0 | 4 |
| pRU2784 (mcpW) | 127 | 2 | 0 | 6 |
| pRU2994 (mcpX) | 417 | 0 | 0 | 6 |
| pRU2898 (mcpY) | 29 | 13 | 12 | 23 |
| pRU2787 (mcpZ) | 154 | 0 | 0 | 17 |
| pRU2250 (icpA = che) | 156 | 0 | 0 | 25 |
a
Transcription from nine chemoreceptor promoters was assessed with plasmid-borne lacZ fusions in wild-type (RU11/001), ΔvisN/R (RU11/814), Δrem (RU11/555), and DflbT (RU13/110) strains during exponential growth. Cells diluted in RB were layered on Bromfield agar plates and grown to an OD600 of 0.15 to 0.25. The che operon (che) is composed of the genes icpA, orf2, cheY1, cheA, cheW, cheR, cheB, cheY2, cheD, and orf10.
b
β-Galactosidase activities (47) of three to five independent experiments were averaged. Standard deviations were between 0.5 and 6%.
c
Values for the wild type were taken from the work of Meier et al. (30).