TABLE 4.
Bacterial strains and plasmids
| Strain/plasmid | Relevant characteristic | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm λ (DE3) | Novagen |
| ER2566 | lon ompT lacZ::T7 | NEB |
| S17-1 | recA endA thi hsdR RP4-2 Tc::Mu::Tn7 Tpr Smr | 60 |
| S. meliloti | ||
| RU11/001 | Smr, spontaneously streptomycin-resistant wild-type strain | 52 |
| RU11/310 | Smr ΔcheA | 61 |
| RU11/555 | Smr Δrem | 43 |
| RU11/803 | Smr ΔmcpW | 30 |
| RU11/804 | Smr ΔmcpY | 30 |
| RU11/805 | Smr ΔmcpX | 30 |
| RU11/814 | Smr ΔvisNR | 44 |
| RU11/815 | Smr ΔicpA | 30 |
| RU11/818 | Smr ΔmcpZ | 30 |
| RU11/828 | Smr ΔmcpU | 30 |
| RU11/830 | Smr ΔmcpV | 30 |
| RU11/838 | Smr ΔmcpT | 30 |
| RU13/142 | Smr mcpT-egfp | 48 |
| RU13/143 | Smr mcpW-egfp | 48 |
| RU13/243 | Smr cheA-egfp | 48 |
| RU13/301 | Smr mcpU-egfp | 48 |
| RU13/303 | Smr icpA-egfp | 48 |
| RU13/310 | Smr ΔflbT | This study |
| Plasmids | ||
| pHT28 | Apr, expression vector for E. coli fliM | 62 |
| pK18mobsacB | Kmr lacZ mob sacB | 63 |
| pKLD66 | Apr, expression vector | 64 |
| pTYB1 | Apr, expression vector | NEB |
| pTYB11 | Apr, expression vector | NEB |
| pBS352 | Apr, 858-bp NdeI/SapI PCR fragment containing periplasmic domain of mcpX cloned into pTYB1 | This study |
| pBS353 | Apr, 741-bp NdeI/SapI PCR fragment containing periplasmic domain of mcpU cloned into pTYB1 | This study |
| pBS409 | Apr, 474-bp SapI/PstI PCR fragment containing periplasmic domain of mcpV cloned into pTYB11 | This study |
| pBS426 | Apr, 1,218-bp SapI/SpeI PCR fragment containing periplasmic domain of mcpZ cloned into pTYB11 | This study |
| pBS487 | Apr, 1,602-bp KpnI/HindIII PCR fragment containing icpA cloned into pKLD66 | This study |
| pBS1030 | Apr, 444-bp NdeI/PstI PCR fragment containing periplasmic domain of mcpT cloned into pTYB1 | This study |
| pBS1031 | Apr, 423-bp NdeI/PstI PCR fragment containing periplasmic domain of mcpW cloned into pTYB1 | This study |
| pRU2250 | Tcr, icpA (1,974 bp)-lacZ (che) fusion cloned into pPHU236 | 30 |
| pRU2782 | Tcr, mcpT (320 bp)-lacZ fusion cloned into pPHU235 | 30 |
| pRU2783 | Tcr, mcpU (456 bp)-lacZ fusion cloned into pPHU236 | 30 |
| pRU2784 | Tcr, mcpW (303 bp)-lacZ fusion cloned into pPHU236 | 30 |
| pRU2787 | Tcr, mcpZ (409 bp)-lacZ fusion cloned into pPHU236 | 30 |
| pRU2790 | Apr, 1,779-bp KpnI/PstI PCR fragment containing mcpY replacing E. coli fliM in pHT28 | 30 |
| pRU2898 | Tcr, mcpY (786 bp)-lacZ fusion cloned into pPHU236 | 30 |
| pRU2994 | Tcr, mcpX (590 bp)-lacZ fusion cloned into pPHU236 | 30 |