Table 1. Mean and standard deviation of thickness calculated for each segmentation method.

Segmentation method	Thickness mean (pixels)	σ (pixels)	Thickness mean (μm)	σ (μm)
RCA.1	5.054	1.340	39.728	10.533
RCA.2	5.026	1.386	39.508	10.895
MIA-Clustering algorithm	5.111	0.952	40.176	7.484

Notes:

RCA.1 used parameters lower threshold: 7,000, upper threshold: 20,000 and minimum edge strength: 5,000; RCA.2 used lower threshold: 18,000, upper threshold: 26,000 and minimum edge strength: 20,000. Note the near identical measurements using two different sets of values. Parameters for the MIA-Clustering algorithm were grid size: 7 and probability threshold 85%.