Fig. 3.

Effect of ectopic LAP2α expression on proliferation of patient cells depends on the levels of nucleoplasmic lamins. (A) Human primary fibroblasts were transfected either with a control GFP-expressing construct (GFP ctrl) or a human myc-LAP2α-expressing construct (myc-LAP2α) on two consecutive days and counted on days 4–8 (n=3). (B) Primary human fibroblasts were fixed with 4% paraformaldehyde and processed for immunofluorescence using anti-lamin A specific antibody. Scale bars: 10 µm. (C) Fluorescence intensity of the lamin A signal was measured across nuclei (dotted line in B) and plotted. *P<0.05, **P<0.005.