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. 2018 Feb 1;131(3):jcs210245. doi: 10.1242/jcs.210245

Fig. 1.

Fig. 1.

Specific motifs in HDS1 regulate Golgi recruitment of GBF1. (A) Domain organization of GBF1, ARNO, BRAG2 and EFA6. The A795E mutation conferring BFA resistance to GBF1 is marked. DCB, dimerization and cyclophilin-binding; HUS, homology upstream of Sec7; HDS1-3, homology downstream of Sec7; CC, coiled coil; PH, pleckstrin homology. (B) Alignment of HDS1 sequences from Homo sapiens GBF1 and orthologs from Gallus gallus (Ggal), Drosophila melanogaster (Dmel), Caenorhabditis elegans (Cele) and Schizosaccharomyces pombe (Spom) shows identical residues in yellow and marked by asterisks. Strongly conserved residues are in dark green and marked by a colon; weakly conserved residues are in light green and marked with a period. The mutated residues are boxed and α-helical regions are marked by red lines. (C) HeLa cells transfected with the indicated constructs were processed for IF with anti-GFP and anti-GM130. Scale bars: 7 µm. (D) Images analogous to those in C were used to quantitate the percentage of each construct co-localizing with GM130. Data from 20 cells expressing each construct are presented as a scatter plot.