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. 2018 Feb 1;131(3):jcs210245. doi: 10.1242/jcs.210245

Fig. 2.

Fig. 2.

Intact LF motif in HDS1 is required for GBF1 recruitment to Golgi. (A–C) HeLa cells expressing the indicated constructs and stained with BODIPY TR C5-ceramide were imaged under live-cell conditions. Scale bars: 10 µm. (D) Quantification of the percentage of total cellular construct that co-localizes with BODIPY indicates a statistically significant difference in targeting of GBF1/795 and GBF1/795/LF (P<0.0001 by simple one-way ANOVA; P<0.05 by t-test). (E) Lysates from HeLa cells expressing the indicated constructs were separated on a blue native gel and western blotted with anti-GFP.