Figure 5.

Cnb1-deficient CD4⁺ T cells produce more interferon (IFN)γ. (A) Proportion of CD4⁺ T cells producing IFNγ, IL-2, IL-17, or IL-4 in colonic-lamina propria (LP) isolated from Cnb1^fl/fl and Cnb1^CD4 mice. (B) Purified naïve CD62L^highCD44^neg CD4⁺ T cells from the spleens of Cnb1^fl/fl and Cnb1^CD4 mice were cultured under conditions to promote Th0, Th1, Th17, Th2, and Treg cells. T cells were collected after 5 days and stimulated with PMA/ionomycin for 5 h, with the addition of Brefeldin A for the final 4 h. Cells were stained for intracellular IFNγ, IL-17, IL-4, and FoxP3, and the proportion of CD4⁺ T cells producing these cytokines was assessed by flow cytometry. Representative flow-cytometric dot plots are shown. Data represent the means ± standard error of two or three independent experiments (n = 3–4 mice per group, per experiment). *P < 0.05; **P < 0.01; ***P < 0.005 (two-tailed, unpaired Student’s t-test). Abbreviations: Th0, naïve T cell; Th1, Type-1 T helper cell; Th2, Type-2 T helper cell; Th17, T helper 17 cell; Treg, regulatory T-cell.