TABLE 2.

Type II binding of triazole drugs to wild-type and mutant ScErg11p6×His enzymes^a

ScErg11p6×His enzyme	Triazole	ΔAmax	Equation	Mean Kd (μM) (SE)	Hill coefficient	IC50 (µM)
WT	FLC	0.044	Hill	0.141 (±0.028)	1.5	0.27
	VCZ	0.036	Hill	0.051 (±0.019)	2	0.23
	ITC	0.033	Hill	0.123 (±0.027)	1.6	0.28
	PCZ	0.034	Hill	0.078 (±0.023)	2.2	0.32
G73E	FLC	0.043	Morrison	0.008 (±0.007)	NA	0.45
	VCZ	0.043	Hill	0.08 (±0.025)	2.5	0.36
	ITC	0.034	Hill	0.07 (±0.04)	1.9	0.25
	PCZ	0.042	Hill	0.10 (±0.04)	2.1	0.36
G73R	FLC	0.030	Hill	0.08 (±0.02)	2.6	0.37
	VCZ	0.028	Hill	0.12 (±0.1)	1.7	0.29
	ITC	0.030	Hill	0.08 (±0.04)	2.2	0.31
	PCZ	0.031	Hill	0.04 (±0.02)	2.3	0.25
G73W	FLC	0.051	Hill	0.47 (±0.097)	2.3	0.71
	VCZ	0.053	Morrison	0.014 (±0.007)	NA	0.51
	ITC	0.047	Morrison	0.023 (±0.009)	NA	0.52
	PCZ	0.047	Morrison	0.005 (±0.006)	NA	0.44

^aThe preferred equation to fit the data was chosen by using the Akaike information criterion (47). Values in parentheses indicate standard errors. WT, wild type; NA, not applicable.