FIG 3.

In vivo activity of sulfa drug-fluconazole combinations on C. elegans infected with C. albicans (NR 29448). Synchronized C. elegans worms, strain AU37 genotype [glp-4(bn2) I; sek-1(km4) X], were infected with C. albicans strain NR 29448 for 3 h and then treated with the indicated drugs. Sulfa drugs were used at 10× MICc in combination with fluconazole (10 μg/ml). 5-Fluorocytosine (5-FC) was used as a positive control. After 24 h of treatment, the worms were washed out and disrupted using silica carbide beads, and then the CFU/worm were counted on YPD plates containing streptomycin-penicillin.