FIG 4.

Effects of different sulfa antibacterial drugs on intracellular accumulation of rhodamine 123 by C. albicans (NR 29448). Deenergized pretreated Candida cells with subinhibitory concentrations of the indicated sulfa drugs (128 μg/ml) were loaded with 10 μM rhodamine for 30 min at 35°C. Then, the cells were washed thrice to remove extracellular dye. Pellets were resuspended in PBS, and fluorescence at excitation and emission wavelengths of 485 and 538 nm, respectively, was recorded. Cyclosporine was used as a positive control.