TABLE 2.

Study of virulence in the Galleria mellonella larvae^a

Isolate no. and echinocandin plate exposure	MIC (mg/liter)		FKS2 HS1 sequence (no. of colonies)	Median survival (days)
	MYC	AND
1
None	0.015	0.03	WT	5
MYC	4	2	ΔF658 (1)	6
	0.25	0.5	E655A (1)	5
	0.06	0.125	WT (1)	5.5
AND	0.06	0.125	WT (6)	4.5
	0.06	0.25	WT (2)	4
2
None	0.015	0.015	WT	5
MYC	0.5	0.5	W715L (1)	5
	2	1	ΔF658 (1)	5
AND	2	2	ΔF658 (1)	5.5
3
None	0.015	0.015	WT	3
MYC	4	4	ΔF658 (1)	3
AND	2	4	ΔF658 (1)	3
4
None	0.015	0.03	WT	3.5
MYC	4	2	ΔF658 (1)	3.5
AND	0.06	0.25	WT (1)	4.5
5
None	0.015	0.03	WT	2
AND	1	1	ΔF658 (1)	2
6
None	0.015	0.03	WT	3
MYC	4	2	ΔF658 (1)	5.5
	4	2	S663P (1)	3.5
7
None	0.015	0.03	WT	2
MYC	2	1	S663P (1)	2
8
None	0.015	0.03	WT	6
AND	4	2	ΔF658 (1)	6.5
	2	2	ΔF658 (1)	6
9
None	0.015	0.03	WT	2.5
AND	1	2	ΔF658 (1)	2.5
10
None	0.015	0.03	WT	4
AND	1	2	ΔF658 (1)	7
	0.06	0.25	WT (1)	4
11
None	0.015	0.03	WT	3
AND	2	2	S663P (1)	3
	4	2	S663P (1)	4
12
None	0.015	0.015	WT	3
MYC	4	2	W715L (1)	3
	2	4	ΔF658 (1)	3.5
AND	4	2	ΔF658 (1)	5

^aResults are larvae infected by 32 colonies (FKS2 mutant and FKS2 wild type) obtained from the 12 isolates streaked onto plates containing 1 mg/liter echinocandin.